Namareq Musa Ibrahim Ahmed on LinkedIn: McFarland standards are essential in microbiology laboratories. They serve… (2024)

Namareq Musa Ibrahim Ahmed

The Second specialist of Medical laboratory sciences especially of Microbiology and Parasitology.Digital Educational creator.namareqmusaibrahim@gmail.com

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McFarland standards are essential in microbiology laboratories. They serve as reference points for adjusting the turbidity of bacterial suspensions, ensuring that the number of bacteria falls within a specific range for standardized microbial testing1. Let’s delve into the details:Purpose: McFarland standards are used primarily for antibiotic susceptibility testing. By measuring the minimum inhibitory concentration, these standards help determine whether bacteria are resistant or susceptible to specific antimicrobial agents1.Original McFarland Standards: The original standards were created by mixing barium chloride and sulfuric acid. The resulting barium sulfate precipitate caused turbidity in the solution.A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl₂•2H₂O) with 9.95 mL of 1% sulfuric acid (H₂SO₄)1.Modern McFarland Standards: Nowadays, McFarland standards are prepared from latex particle suspensions, which enhance shelf life and stability.These standards can be visually compared to bacterial suspensions in sterile saline or nutrient broth.Adjustments can be made: If the bacterial suspension is too turbid, dilute it with more diluent.If it’s not turbid enough, add more bacteria1.McFarland Nephelometer Standards: Here’s a comparison of different McFarland standards: No. 0.5: 1.5 × 10⁸ CFU/mL, 74.3% transmittance, absorbance at 600 nm: 0.08 to 0.1No. 1: 3.0 × 10⁸ CFU/mL, 55.6% transmittance, absorbance at 600 nm: 0.257No. 2: 6.0 × 10⁸ CFU/mL, 35.6% transmittance,absorbance at 600 nm: 0.451No. 3: 9.0 × 10⁸ CFU/mL, 26.4% transmittance, absorbance at 600 nm: 0.582No. 4: 12.0 × 10⁸ CFU/mL, 21.5% transmittance, absorbance at 600 nm: 0.6691Remember, McFarland standards play a crucial role in ensuring accurate microbial testing results!#Microbiology #MIC

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  • Pritam Ghosh

    Executive, Microbiology, Quality Operations at Square Pharmaceuticals PLC.,Bangladesh.

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    * Few insights about McFarland standards!McFarland standardsare essential in microbiology laboratories.They serve as reference points for adjusting theturbidityof bacterial suspensions, ensuring that the number of bacteria falls within a specific range for standardized microbial testing.Let’s see into the details:Purpose: McFarland standards are used primarily forantibiotic susceptibility testing or BioAssay.By measuring theminimum inhibitory concentration, these standards help determine whether bacteria are resistant or susceptible to specific antimicrobial agents. It’s also used to make standard bacterial preparations for Analytical Profile Index identification methods.Original McFarland Standards: The original standards were created by mixingbarium chlorideandsulfuric acid. The resultingbarium sulfate precipitatecaused turbidity in the solution.A0.5 McFarland standardis prepared by mixing0.05 mLof1.175% barium chloride dihydrate(BaCl₂•2H₂O) with9.95 mLof1% sulfuric acid(H₂SO₄).Modern McFarland Standards: Nowadays, McFarland standards are prepared fromlatex particle suspensions, which enhance shelf life and stability.These standards can be visually compared to bacterial suspensions in sterile saline or nutrient broth.Adjustments can be made as if the bacterial suspension is too turbid, dilute it with more diluent.If it’s not turbid enough, add more bacteria.McFarland Nephelometer Standards: Here’s a comparison of different McFarland standards: No. 0.5: 1.5 × 10⁸ CFU/mL, 74.3% transmittance, absorbance at 600 nm: 0.08 to 0.1No. 1: 3.0 × 10⁸ CFU/mL, 55.6% transmittance, absorbance at 600 nm: 0.257No. 2: 6.0 × 10⁸ CFU/mL, 35.6% transmittance, absorbance at 600 nm: 0.451No. 3: 9.0 × 10⁸ CFU/mL, 26.4% transmittance, absorbance at 600 nm: 0.582No. 4: 12.0 × 10⁸ CFU/mL, 21.5% transmittance, absorbance at 600 nm: 0.6691Remember, McFarland standards play a crucial role in ensuring accurate microbial testing results!

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  • Heba Awadallah

    Quality control Microbiologist MBA in health care , TQM and 6 Sigma Certified ,microbiological analysis and environmental control

    McFarland standardsare essential in microbiology laboratories.They serve as reference points for adjusting theturbidityof bacterial suspensions, ensuring that the number of bacteria falls within a specific range for standardized microbial testing1. Let’s delve into the details:Purpose: McFarland standards are used primarily forantibiotic susceptibility testing.By measuring theminimum inhibitory concentration, these standards help determine whether bacteria are resistant or susceptible to specific antimicrobial agents1.Original McFarland Standards: The original standards were created by mixingbarium chlorideandsulfuric acid. The resultingbarium sulfate precipitatecaused turbidity in the solution.A0.5 McFarland standardis prepared by mixing0.05 mLof1.175% barium chloride dihydrate(BaCl₂•2H₂O) with9.95 mLof1% sulfuric acid(H₂SO₄)1.Modern McFarland Standards: Nowadays, McFarland standards are prepared fromlatex particle suspensions, which enhance shelf life and stability.These standards can be visually compared to bacterial suspensions in sterile saline or nutrient broth.Adjustments can be made: If the bacterial suspension is too turbid, dilute it with more diluent.If it’s not turbid enough, add more bacteria1.McFarland Nephelometer Standards: Here’s a comparison of different McFarland standards: No. 0.5: 1.5 × 10⁸ CFU/mL, 74.3% transmittance, absorbance at 600 nm: 0.08 to 0.1No. 1: 3.0 × 10⁸ CFU/mL, 55.6% transmittance, absorbance at 600 nm: 0.257No. 2: 6.0 × 10⁸ CFU/mL, 35.6% transmittance, absorbance at 600 nm: 0.451No. 3: 9.0 × 10⁸ CFU/mL, 26.4% transmittance, absorbance at 600 nm: 0.582No. 4: 12.0 × 10⁸ CFU/mL, 21.5% transmittance, absorbance at 600 nm: 0.6691Remember, McFarland standards play a crucial role in ensuring accurate microbial testing results!

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  • Noureldin Mostafa

    Quality Assurance Section Head

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    McFarland standardsare essential in microbiology laboratories.They serve as reference points for adjusting theturbidityof bacterial suspensions, ensuring that the number of bacteria falls within a specific range for standardized microbial testing1. Let’s delve into the details:Purpose: McFarland standards are used primarily forantibiotic susceptibility testing.By measuring theminimum inhibitory concentration, these standards help determine whether bacteria are resistant or susceptible to specific antimicrobial agents1.Original McFarland Standards: The original standards were created by mixingbarium chlorideandsulfuric acid. The resultingbarium sulfate precipitatecaused turbidity in the solution.A0.5 McFarland standardis prepared by mixing0.05 mLof1.175% barium chloride dihydrate(BaCl₂•2H₂O) with9.95 mLof1% sulfuric acid(H₂SO₄)1.Modern McFarland Standards: Nowadays, McFarland standards are prepared fromlatex particle suspensions, which enhance shelf life and stability.These standards can be visually compared to bacterial suspensions in sterile saline or nutrient broth.Adjustments can be made: If the bacterial suspension is too turbid, dilute it with more diluent.If it’s not turbid enough, add more bacteria1.McFarland Nephelometer Standards: Here’s a comparison of different McFarland standards: No. 0.5: 1.5 × 10⁸ CFU/mL, 74.3% transmittance, absorbance at 600 nm: 0.08 to 0.1No. 1: 3.0 × 10⁸ CFU/mL, 55.6% transmittance, absorbance at 600 nm: 0.257No. 2: 6.0 × 10⁸ CFU/mL, 35.6% transmittance, absorbance at 600 nm: 0.451No. 3: 9.0 × 10⁸ CFU/mL, 26.4% transmittance, absorbance at 600 nm: 0.582No. 4: 12.0 × 10⁸ CFU/mL, 21.5% transmittance, absorbance at 600 nm: 0.6691Remember, McFarland standards play a crucial role in ensuring accurate microbial testing results!

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  • Cherwell Laboratories Ltd

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    Dive into our latest article to understand how colour variations in prepared media can impact your microbiological results. Cherwell provides insights and solutions to ensure consistency and reliability in your lab work. #Cherwell #PreparedMedia #Microbiology https://bit.ly/3XEwHYr

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  • Mariya Hamid

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    Medical MicrobiologyGram Staining:Gram staining is a microbiological technique that distinguishes bacteria based on cell wall properties. Bacteria are first stained with crystal violet, fixed with iodine, then decolorized with alcohol or acetone. Gram-positive bacteria retain the purple stain due to a thick peptidoglycan layer, while gram-negative bacteria, with a thinner peptidoglycan layer, appear red or pink after counterstaining with safranin. This method is crucial for identifying bacteria and guiding antibiotic treatment.

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    "CHROMagar™ ECCis the perfect solution for the simultaneous detection and enumeration ofE. coliand other coliforms in food and water samples."https://lnkd.in/dZwjhtqV#wateranalysis #watertesting #pharmaceuticalindustry #pharma #pharmaindustry #microbiology #antibioticresistance #health #medicalscience #microbiology #foodmicrobiology #hospital #bacteriology #clinicallaboratory #clinicalresearch #medicine #medicalsciences #scienceandenvironment

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    Bacterial count is a crucial aspect of microbiological analysis as it helps determine the number of viable bacteria present in a given sample. The colony-forming unit (CFU) assay is the most commonly used method for bacterial count. In this method, the sample is plated on agar media that supports bacterial growth and individual bacterial cells form visible colonies. These colonies represent a single viable bacterium or a group of bacteria derived from a single cell. Once the colonies have developed, they are counted and multiplied by the dilution factor to determine the bacterial count in the original sample. The count is usually expressed as CFU per milliliter (CFU/mL) or CFU per gram (CFU/g) of the sample. This method is based on the assumption that each viable bacterium can give rise to a single colony under suitable conditions. Understanding bacterial count is essential for ensuring the safety and quality of various products, including food and pharmaceuticals. #Microbiology #BacterialCount #CFUAssay #BacterialCount #MicrobialLoad #FoodSafety#EnvironmentalMonitoring#Healthcare#MicrobialAnalysis #ColonyFormingUnit#CFU#MicrobialQuality #HygienePractices

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